READGEN

Real-time adaptation of single cells to genetic changes and implications for bioprocesses



Résumé
The way by which mutations allow living organisms to adapt their physiology is complex, dynamic and heterogeneous. It is also a fundamental process of natural evolution which can be artificially applied to improve the yields of industrial bioprocesses. We propose to exploit two technological developments (one from biology and one from physical-chemistry) that, when combined together, have the potential to monitor the phenotype of individual de novo mutant cells, in real time and at very high throughput. This development can in principle have wide applications in biology. By using it on yeast cells, we expect to reveal fine-scale properties of a basic evolutionary force (the spectrum of phenotypic possibilities generated by mutations) and to describe metabolic burden (a natural limitation of bioproduction) at unprecedented resolution.

Mots-clés
Génétique évolutive, Optogénétique, Bioprocédé, Biotechnologie, Analyse cellules uniques

Partenaires du projet

INSB
YVERT Gael
(UMR5239) Lyon France
INC
BAUDRY Jean
Laboratoire Colloïdes et Matériaux Divisés (UMR8231) France
Arthur Goldstein
Crédit photo : Arthur Goldstein

Pour plus d’informations http://www.ens-lyon.fr/LBMC/gisv/