PhagoChemiForce

Disentangling phagocytosis complexity using chemically multiresponsive and deformable particles



Résumé
Phagocytosis is a mechanism of internalization of objects larger than 0.5 microns. How multiple receptors simultaneously recognize microbes, leading to a complex interplay between the signaling pathways and a fine-tuning of the fate of the internalised material, is still not well understood. This proposal aims at untangling critical steps of phagocytosis in macrophages taking advantage of a multi-disciplinary approach and unique deformable emulsion droplets coated with novel fluorescent receptor-targeted ligands to detect by FRET receptors binding and clustering, and to monitor directly the forces generated by the phagocyte. The project will focus on receptors for important pathogenic microbes, which have been overlooked so far, mannose receptors, and their cross talk with complement receptors. Important regulators of force generation and phagosome formation will then be identified in primary macrophages using these unique read-outs.

Mots-clés
deformable lipid droplets, fluorescent probes, macrophages, mechanobiology, phagocytosis

Partenaires du projet

INSB
NIEDERGANG Florence
(UMR8104) Paris France
INC
FATTACCIOLI JACQUES
Laboratoire microfluidique Institut Pierre Gilles de Gennes – CNRS UMR 8640- INC, ENS, Sorbonne Université (UMR8640) France
INC
MALLET JEAN-MAURICE
Laboratoire des Biomolécules, CNRS UMR7203- INC ENS, Sorbonne Université (UMR7203) France
@Laura Jacquelin
Crédit photo : @Laura Jacquelin