CageForMap

High throughput functional description of cerebellar networks using new caged neurotransmitters



Résumé
The goal of this project is to bring the proof of concept that the map of functional synaptic organization of any cerebral modules - i.e. the excitatory and inhibitory synaptic inputs of the principal cells - can be quickly described using systematic scanning of cerebellar slices with 3D photo-stimulation of a new family of caged compounds. First, we (chemist team) will design and test new families of caged compounds with an increased hydrolytical stability, high blue light and IR two-photon sensitivity and optimal pharmacological properties. Second, we (cerebellum team) will describe the synaptic maps of cerebellar cortical modules which have specific regional properties using two-photon excitation of caged neurotransmitters and neuromodulators in small volume of tissue. We will unravel the operating modes of cerebellar modules and bring new hypothesis on how they process, combine and communicate information.

Mots-clés
Caged neurotransmitter, cerebellum, synaptic map, , two-photon excitation

Partenaires du projet

INSB
ISOPE Philippe
Laboratoire de Conception et Application de Molécules Bioactives (UPR3212) Strasbourg France
INC
SPECHT Alexandre
Laboratoire de Conception et Application de Molécules Bioactives (UMR7199) France
et l'image attachée representant les differents types cellulaires dans une tranche de cerveletCredit: c'est une photo de l'équipe que nous avons donné à la photothèque du CNRS: Jean-Luc Dupont/INCI/CNRS
Crédit photo : Differents types cellulaires dans une tranche de cervelet. Credit: Jean-Luc Dupont/INCI/CNRS