BAMS

Bacteria meet surfaces: how the micromechanical environment impacts bacterial virulence



Résumé
Bacteria adhere to surfaces where they form macrocolonies called biofilms. However, the cues that regulate the switch from an individual motile lifestyle to a collective one are not clear. Building on recent evidence, we propose to study whether the bacterial mechanical microenvironment influences this transition. Using a cross-disciplinary approach, we will image bacteria in flow cells in situ as they adhere on mechanically controlled substrates, which rigidity can be tuned from 1-100kPa. Combining imaging and transcriptome sequencing approaches, we will investigate the correlation between mechanical cues, bacterial organization and virulence regulation in early surface colonization.

Mots-clés
bacterial adhesion, biofilm, surface-sensing, mechano-sensing, motility

Partenaires du projet

INP
LECUYER Sigolene
(UMR5672) Lyon France
INSB
Attree Ina
Biologie du cancer et de l'infection (BCI) (ERL5261) France
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